Part:BBa_K2985004
ycxA->ycxA gene encodes a specific carrier protein. In the process of cell growth, YcxA protein effe
The ycxA gene is located in surfactin synthase gene cluster downstream of srfA operon. It encodes proteins homologous to members of the main promoter superfamily. It is mainly related to active transport of lipopeptides. The length of ycxa coding region is 1227 bp. According to Xu, ycxA protein is mainly a transmembrane protein, which participates in the export of surfactin and trans-proton gradient transport mechanism. Overexpression of ycxA gene resulted in a marked increase in surfactin production. Meanwhile, hemolytic circles were observed in single cells cultured on agar plates. If you want to use it in the project, you can get it from the genome of most wild-type Bacillus subtilis.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 905
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 905
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 905
Illegal BglII site found at 816 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 905
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 905
Illegal NgoMIV site found at 484 - 1000COMPATIBLE WITH RFC[1000]
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