Part:BBa_K2922026

Lysis protein for colicin-E1 coding region

Summary

Abbreviated as Ekil, lysis protein for Colicin-E1 is a 4.8 kDa protein, which is encoded by gene kil.The lysis protein is required for both Colicin-E1 release and partial cell lysis. [1] (Fig.1)

Fig.1 Ekil helps Colicin-E1 release and makes partial cell lysis.

Identification

When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify. After the circuit was built, we sent the plasmid to sequence, and got the correct sequencing. After new molecular cloning experiments, we did Enzyme-Cut identification to certify the plasmid was correct. We used the XbaI and PstI to cut the plasmid, then we got the target separate fragment-138bp. (Fig.2)

Fig.2 The result of this plasmid cut with enzyme XbaI and PstI.

Reference

[1] E. Cascales et al., Colicin biology. Microbiol Mol Biol Rev 71, 158-229 (2007).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 118
  
• 1000
  COMPATIBLE WITH RFC[1000]