Composite

Part:BBa_K3120018

Designed by: Shuiqin Gong   Group: iGEM19_BM-AMU   (2019-08-14)
Revision as of 08:22, 14 August 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


E-cad LA+Glycine linker+tdTomato+E-cad RA

This part is the homologous recombination plasmid of E-Cadherin. Using crisper CAS9 technology, homologous recombination can occur between the left arm and right arm of E-Cadherin and the corresponding fragments on the genome, so as to realize the insertion of tdTomato before the termination codon of E-Cadherin gene of homo sapiens H9(human embryonic stem cell line).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3994
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 333
    Illegal BsaI site found at 782
    Illegal BsaI site found at 4472
    Illegal BsaI site found at 4635
    Illegal BsaI site found at 4658
    Illegal BsaI.rc site found at 1647


[edit]
Categories
Parameters
None