Coding
xis

Part:BBa_K112001:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-20)
Revision as of 01:40, 21 October 2008 by Registry (Talk | contribs)

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Xis from bacteriophage lambda, assembly standard 21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112001

bx3 Forward biobricking of {<T4 Holin!} ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc bx2 Reverse biobricking of {<T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc </pre>

References