Composite

Part:BBa_K2957034

Designed by: Malik Aaron George   Group: iGEM19_MIT   (2019-10-19)
Revision as of 23:17, 19 October 2019 by Registry (Talk | contribs)

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hEF1a, IL-8 (CXCL-8)

Contents: TRE promoter with C5a Gene fused to NeonGreen fluorescent protein, Carb resistance What is it?: A Composite part; we call it a pL1 What does it do?: It codes for the chemokine, C5a, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of neutrophil. How to use it?: This part was created with Type IIS Cloning and transfected into HEKs. We tested it with HL-60s. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3667
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2684
    Illegal BamHI site found at 3290
    Illegal BamHI site found at 3569
    Illegal XhoI site found at 3083
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3667
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3667
    Illegal PstI site found at 2430
    Illegal PstI site found at 2935
    Illegal NgoMIV site found at 197
    Illegal NgoMIV site found at 2818
    Illegal AgeI site found at 2025
    Illegal AgeI site found at 2196
    Illegal AgeI site found at 3673
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2073
    Illegal SapI.rc site found at 9


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