Composite

Part:BBa_K3105680:Design

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-12)
Revision as of 23:20, 19 October 2019 by JonasG (Talk | contribs) (→‎Design Notes)


Expression construct AAO-2A-eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1356
    Illegal BamHI site found at 1543
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2503
    Illegal AgeI site found at 3896
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was codon optimised for Pichia pastoris
eGFP was added to the C-terminal to develop an easy method to identify AAO-expressing P. patoris clones.

Source

see basic parts

References