Composite
Part:BBa_K3105680:Design
Designed by: Jonas Gockel Group: iGEM19_Uppsala_Universitet (2019-10-12)
Expression construct AAO-2A-eGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1356
Illegal BamHI site found at 1543
Illegal XhoI site found at 1183 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2503
Illegal AgeI site found at 3896 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was codon optimised for Pichia pastoris
Source
see basic parts