Part:BBa_K2957007

hEF1a, IL-8 (CXCL-8)

Contents: hEF1a, IL-8 (CXCL-8), NeonGreen Tag, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression

What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. It also has a NeonGreen tag attached to the IL8 so this allows us to visualize it and determine transfection efficiency. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 3667
  Illegal PstI site found at 2430
  Illegal PstI site found at 2935
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 2430
  Illegal PstI site found at 2935
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2684
  Illegal BamHI site found at 3290
  Illegal BamHI site found at 3569
  Illegal XhoI site found at 3083
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 3667
  Illegal PstI site found at 2430
  Illegal PstI site found at 2935
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 3667
  Illegal PstI site found at 2430
  Illegal PstI site found at 2935
  Illegal NgoMIV site found at 197
  Illegal NgoMIV site found at 2818
  Illegal AgeI site found at 2025
  Illegal AgeI site found at 2196
  Illegal AgeI site found at 3673
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 2073
  Illegal SapI.rc site found at 9