Part:BBa_K2957031
TRE, CCL5
Contents: TRE promoter with CCL5 Gene, Carb resistance What is it?: A Composite part; we call it a pL1 What does it do?: It codes for the chemokine, CCL5, which is involved in immune response. It was important for our project in particular because of its involvement in the immune system and is known to signal chemotaxis of T-cells. How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2943
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2943
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2943
Illegal NgoMIV site found at 1479
Illegal AgeI site found at 2949 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2623
Illegal BsaI.rc site found at 2921
Illegal SapI site found at 402
Illegal SapI.rc site found at 2560
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