Part:BBa_K2929004

mOrange + SpyTag

mOrange (BBa_E2050) conjugated to SpyTag (BBa_K1159201) via a 2xGGSG Linker. mOrange is a flourescent protein derived from RFP in Discosoma sp., and SpyTag is an oligopeptide which ligates to a SpyCatcher binding partner via an isopeptide bond. SpyTag is derived from the surface proteins of Streptococcus pyogenes.

This part is an improvement over BBa_E2050 as it is codon-optimised for Escherichia coli, and possesses a SpyTag which allows ligation to other proteins via a SpyCatcher domain. We do not predict the SpyTag domain to impact the expression or fluorescence of this part, so we believe BBa_K2929004 to be suitable reporter in E. coli.

Protein Mass: 26.7kDa Protein Excitation: 548nm Protein Emission: 562nm

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 820
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 594
  Illegal AgeI site found at 706
  
• 1000
  COMPATIBLE WITH RFC[1000]