Coding
SP 20 B5-B

Part:BBa_K2984016

Designed by: Sophia Schiebler   Group: iGEM19_Humboldt_Berlin   (2019-10-14)
Revision as of 20:34, 18 October 2019 by Chlamy Dima (Talk | contribs)


SP20 B5-B5 - Glycomodule for enhancement of secretion in C. reinhardtii

This part is a part of the Chlamy-HUB-Collection. It has previously been shown that the fusion of a glycomodule at the C-terminal end of a protein enhances the secretion up to 12-fold and also increases the proteolytic protein stability (Ramos-Martinez et al., 2017). A tandem serine and proline of 20 repeats (SP20) module was therefore created.

This part was designed to be used with the MoClo standard and has B5-B5 overhangs.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Ramos‐Martinez, E. M., Fimognari, L., & Sakuragi, Y. (2017). High‐yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii. Plant biotechnology journal, 15(9), 1214-1224.
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Parameters
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