Composite

Part:BBa_K2922017

Designed by: Zinuo Huang   Group: iGEM19_XMU-China   (2019-10-13)
Revision as of 11:59, 13 October 2019 by Registry (Talk | contribs)

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T7-RBS-cenA (Endoglucanase A) functions in Kil secretion cassette with constitutive promoter J23114

This part contains the sequence for the protein kil regulated by constitutive promoter J23114 and the sequence for the protein endoglucanase A regulated by T7 promoter. We used this part to achieve the secretion of endoglucanase with the function of kil secretion cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 328
    Illegal NotI site found at 1472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1385
    Illegal BamHI site found at 521
    Illegal XhoI site found at 883
    Illegal XhoI site found at 1132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 663
    Illegal NgoMIV site found at 1588
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 567


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Categories
Parameters
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