Coding

Part:BBa_K2969023

Designed by: Liangchen Zhuo   Group: iGEM19_UCAS-China   (2019-10-15)
Revision as of 15:55, 15 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Doc

Doc is a protein usually used in bacteria toxin-antitoxin system (TA system). This TA system is derived from the bacteriophage P1, which lysogenizes in Escherichia coli cells as a stable low-copy plasmid. Doc plays a role of toxin. It is interacted with 30S ribosomal subunits, stabilized polysomes, and resulted in a significant increase in mRNA half-life. So, doc expression resulted in rapid cell growth arrest and marked inhibition of translation without significant perturbation of transcription or replication.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None