Composite

Part:BBa_K2973000

Designed by: Nick Delkis   Group: iGEM19_Thessaly   (2019-08-17)
Revision as of 13:09, 19 October 2019 by Akatsaouni (Talk | contribs)


T7-RBS-β-Lactamase-No Signal peptide

This composite part consists of T7 Promoter(BBa_J64997) and T7 Terminator(BBa_K731721), Ribosomal Binding Site(BBa_B0034), CDS of β-lactamase without the signal peptide. β-lactamase (EC 3.5.2.6) is a small monomeric enzyme(29kDa) that is produced by bacteria and gives them resistance to antibiotics with β-lactam ring because of its ability to hydrolyze the amide bond in the β-lactam ring. This ability can be exploited in order to use β-lactamase as a protein reporter by providing the enzyme with its chromogenic substrate Nitrocefin. Nitrocefin is a chromogenic cephalosporin first reported in 1972 as a novel and straightforward substrate used to detect bacteria resistant to β-lactam antibiotics. Normally, a nitrocefin solution has a yellow color, but after its hydrolysis by β-lactamase, the color of the solution turns red, allowing that way the detection of the enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 851
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None