Regulatory

Part:BBa_K3142000

Designed by: Linsen Huang   Group: iGEM19_SZPT-CHINA   (2019-09-28)
Revision as of 17:31, 13 October 2019 by Xiaolin (Talk | contribs)


Acid-induced promoter（PrcfB）

The rcfB promoter, a member of the CRP-FNR positive regulation family, was amplified from chromosomal DNA lactis subsp. lactis IL1403. It is an important part of the P170 promoter regulated by acid. When the external pH value declines to 5.5, rcfB promoter will be highly upregulated.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
  • 1000
    COMPATIBLE WITH RFC[1000]


Results

The A11Sg-AHPM protein was carried out in the lactococcus lactis MG1363 harboring the pMG36e-rcfB-O1/O2-SPusp45-A11Sg-AHPM vector, which was grown in the GM17 broth under different pH. In the 12%SDS-PAGE, one band of size 64 kDa corresponding to the recombinant modified protein was detected at the pH=6.

PH01.png
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Parameters
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