Composite

Part:BBa_K2922008

Designed by: Tianyu Feng   Group: iGEM19_XMU-China   (2019-10-13)
Revision as of 11:35, 13 October 2019 by Registry (Talk | contribs)

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Expression system of YebF-Cex coding YebF-Exoglucanase with T7 promoter

This part contains the sequence for the protein exoglucanase with protein YebF fused to its N-terminus by GS Linker. We use T7 promoter and RBS related to achieve the high level secretion of exoglucanase with the function of YebF protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 940
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 573
    Illegal NgoMIV site found at 946
    Illegal NgoMIV site found at 1448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 993
    Illegal SapI.rc site found at 1076


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Categories
Parameters
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