Composite

Part:BBa_K3081002

Designed by: Le Zhang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 17:49, 16 October 2019 by Registry (Talk | contribs)

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pBAD-dCas9-linker-sfGFP

This part consists of araC/araBAD promoter and sfGFP fused to the C terminus of dCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2321
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 5369


[edit]
Categories
Parameters
None