Composite

Part:BBa_K2922007

Designed by: Tianyu Feng   Group: iGEM19_XMU-China   (2019-10-13)
Revision as of 11:32, 13 October 2019 by Registry (Talk | contribs)

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Expression system of YebF-CenA coding YebF-Endoglucanase A with T7 promoter

This part contains the sequence for the protein endoglucanase A with protein YebF fused to its N-terminus by GS Linker. We use T7 promoter and RBS related to achieve the high level secretion of Endoglucanase A with the function of YebF protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 450
    Illegal NotI site found at 1594
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1507
    Illegal BamHI site found at 643
    Illegal XhoI site found at 1005
    Illegal XhoI site found at 1254
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1710
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 689


[edit]
Categories
Parameters
None