Part:BBa_K3196018

AOX1-Kozak-PHO1-pelA-His tag-AOX1 Terminator

PHO1 is one of three repressible acid phosphatases, a glycoprotein that is transported to the cell surface. pelA can catalyze pectin.

Characterization

This is a four section for degrade and transfer lignin part.

Figure1. T--HUST--China--2019-PHO1pelA

DNA Gel Electrophoretic

After we link PHO1 and pelA successfully, we run the PCR with an intention to confirm the expression of pelA. As the figure shows, we get the genetic stripe about xxx bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.

SDS-PAGE

We run the SDS-PAGE to check whether PHO1 help the enzyme transfer to the extracellular. As the figure shows, we get the protein type about xxx bp which means the SDS-PAGE is successful.

Figure1. This figure shows the most possible kozak consensus sequence.

Enzyme Activity

We use DNS to detect the enzyme activity. As the figure shows, the solution turns xxx, which confirm the enzyme activity.

Figure1. This figure shows the most possible kozak consensus sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1767
  Illegal BamHI site found at 937
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]