Coding

Part:BBa_K2963004

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-07)
Revision as of 14:49, 19 October 2019 by Chikaijun (Talk | contribs)


racE - a glutamate racemase from Bacillus sp.

This part contains the racE gene encoding racemase from Bacillus sp.. This enzyme mainly catelyses the reation of L-glutamate to D-glutamate. In our project, we coexpress the racE with capB*CA complex in order to produce poly-γ-glutamic acid with different ratio of L-glutamic acid.

Usage and Biology

The racE gene is derived from Bacillus subtilis and it encodes a racemase which can converts L-glutamate to D-glutamate.

Characterization

We used enzyme activity data to prove the usability of this part. We measured the enzymatic activity under different tac promoters.

Figure2-gaohaixin.png

We tested the enzyme activity of the racemase in cells. After induction by IPTG, the results showed that the activity of racemase could be detected under the control of different tac promoters, indicating that this part could be used. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None