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Part:BBa_K2983011

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-11)
Revision as of 13:19, 19 October 2019 by Anthor91 (Talk | contribs)


Loop F-Beta

This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.

Usage and Biology

The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the loop Alpha is as fellow : GGTCTCACGCTAGCATGAAGAGC

Figure1: Loop F-Beta structure


Blue triangle: SapI fixation site (GAAGAGC)

Red triangle : BsaI fixation site (GGTCTC)

Blue circle : overhang formed by SapI after cutting (GCA)

Yellow circle: overhang formed by BasI after cutting (CGCT)



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal SapI.rc site found at 17


[edit]
Categories
Parameters
None