Coding
Part:BBa_K3015006
Designed by: Valentina Stuchlik Group: iGEM19_BOKU-Vienna (2019-10-14)
T7 RNA Polymerase (No BbsI/BpiI)
Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, we decided to remove those recognition sites by codon swapping from the Part BBa_K14001 to improve the applicability.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |