Plasmid_Backbone
Level 1 (R

Part:BBa_K2984002

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-12)
Revision as of 13:48, 17 October 2019 by Chlamy Dima (Talk | contribs)


Level 1 c Backbone (RFP-AmpR), Modular Cloning

This vector is a part of the Chlamy-HUB-Collection. E.coli backbone designed for Modular Cloning. Contains red fluorescent protein (RFP) flanked by BpiI recognition sites and ampicillin resistance for screening.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 705
    Illegal XhoI site found at 697
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal AgeI site found at 1511
    Illegal AgeI site found at 1623
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1799
    Illegal BsaI.rc site found at 728


Results

colonies_total
Fig.1 - Transformation of the L1c plasmid in E. coli. The red E. coli colonies obtained the plasmid containing RFP as an selection marker.

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Categories
Parameters
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