Coding

Part:BBa_K2924035:Design

Designed by: Andreas Nakielski   Group: iGEM19_Duesseldorf   (2019-10-11)
Revision as of 11:07, 19 October 2019 by AndreasN (Talk | contribs) (→‎Design Notes)


mVenus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon-optimized for Synechocystis sp. PCC 6803. It was provided by Anna Behle1.

References

Kremers, Gert-Jan, et al. "Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius." Biochemistry 45.21 (2006): 6570-6580.

Behle, Anna, Pia Saake, and Ilka M. Axmann. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.

https://www.fpbase.org/protein/mvenus/