Coding
NeoR

Part:BBa_K1313004

Designed by: Carlos Meza   Group: iGEM14_ITESM-CEM   (2014-10-12)
Revision as of 05:39, 12 October 2014 by Registry (Talk | contribs)

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Neomycin Resistance

Neomycin resistance gene. This gene can be used for selection of cells with an efficient transformation. This gene codes neomycin phosphotransferase which breaks neomycin, kanamycin, and gentamicin molecules. If you use these gene as selectivity gene in bacteria, and your laboratory lacks neomycin, you can replace it for kanamycin by adding 35 mg/ml per liter of LB media. The best concentration of neomycin to use is 80 mg/ml per liter of LB media.

This gene can be also used as a selective gene for mammalian cells using gentamicin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 165
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 165
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 165
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 165
    Illegal NgoMIV site found at 616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 465
    Illegal SapI.rc site found at 675


[edit]
Categories
//cds/selectionmarker/antibioticresistance
Parameters
efficiencyWorks better between 100ug/ml and 150ug/ml of antibiotic per liter of LB media