Composite

Part:BBa_K3081006

Designed by: Yiwen Wang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 09:03, 19 October 2019 by Lelezhang (Talk | contribs)


pBAD-dCas9-J23119-polyA

This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, in which polyA sequence is the control sgRNA targeted no binding site of E.coli genome replication initiation region, OriC.

For more detailed information, see https://parts.igem.org/Part:BBa_K3081058

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None