Coding

Part:BBa_K3165013:Design

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-16)
Revision as of 23:27, 16 October 2019 by Registry (Talk | contribs)

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i^2mCherry (Ile)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Single base-pair mutation at the 48th position and codon optimised for E. coli


Source

The part is derived from the existing mCherry sequence in the iGEM registry (BBa_J18932).

References