Composite

Part:BBa_K3114024

Designed by: Christian Emond, Michael Wahba, Michaella Atienza, Andrew Symes   Group: iGEM19_Calgary   (2019-10-08)
Revision as of 01:03, 19 October 2019 by Mwahba (Talk | contribs)


Chlorophyll B Reductase (CBR) inducible circuit


Usage and Biology

Chlorophyll B Reductase is a naturally occurring plant enzyme, encoded by the gene NYC1, which catalyzes the first step of chlorophyll b degradation (Horie et al. 2009). With the cofactor NADPH, chlorophyll b reductase converts chlorophyll b into 7-Hydroxymethyl Chlorophyll a.

BBa_K3114024 is a part consisting of multiple components of iGEM Calgary's 2019 Biobrick submission kit. The coding region consists of the gene for Chlorophyll b reductase (BBa_K3114008). Following the coding region is

Sequences and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Horie, Y., Ito, H., Kusaba, M., Tanaka, R., & Tanaka, A. (2009). Participation of chlorophyll b reductase in the initial step of the degradation of light-harvesting chlorophyll a/b-protein complexes in Arabidopsis. Journal of Biological Chemistry, 284(26), 17449-17456.




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