Coding

Part:BBa_K3037002:Design

Designed by: Arnau Pérez Roig   Group: iGEM19_TU_Dresden   (2019-10-03)
Revision as of 16:20, 3 October 2019 by Registry (Talk | contribs)

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dead CRISPR Associated Protein (dCas9)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1096
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- Mutated EcoRI site in the midde of the coding region by site directed mutagenesis PCR



Source

Synthesized by Integrated DNA Technologies (IDT)

References