Part:BBa_K3114010

Magnesium Dechelatase Stay Green (SGR)

The conversion of chlorophyll a to pheophytin a is the first step in the natural chlorophyll degradation pathway. The enzyme Magnesium Dechelatase, encoded by the SGR gene, catalyzes this reaction by extracting magnesium (Mg²⁺) from chlorophyll, along with cofactors 2H⁺ (Shimoda et al. 2016)

Usage and Biology

BBa_K3114010 is a golden gate compatible part which allows insertion of the SGR gene into genetic circuits to express the enzyme magnesium dechelatase.

Collection of Parts

This part was designed to be Golden Gate compatible with certain Golden Gate parts in iGEM Calgary's 2019 part collection including the following:
1. DsbA signal peptide - Bba_K3114000 https://parts.igem.org/Part:BBa_K3114000
2. MalE signal peptide - Bba_K3114001 https://parts.igem.org/Part:BBa_K3114001
3. OmpA signal peptide - Bba_K3114002 https://parts.igem.org/Part:BBa_K3114002
4. PhoA signal peptide - Bba_K3114003 https://parts.igem.org/Part:BBa_K3114003
5. Tat signal peptide - Bba_K3114004 https://parts.igem.org/Part:BBa_K3114004
6. TorA signal peptide - Bba_K3114005 https://parts.igem.org/Part:BBa_K3114005
7. Double Terminator - Bba_K3114013 https://parts.igem.org/Part:BBa_K3114013

References

Shimoda, Y., Ito, H., & Tanaka, A. (2016). Arabidopsis STAY-GREEN, Mendel’s green cotyledon gene, encodes magnesium-dechelatase. The Plant Cell, 28(9), 2147-2160.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 4
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1508
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1
  Illegal BsaI.rc site found at 1883