Part:BBa_K3071006
Phage shock protein F (PspF)
PspF is a constitutively active enhancer-binding protein for activating the PspA operon transcription in vivo. The enhancer is located in -80 to -126 upstream of the pspA transcription start site. It has a hexameric structure, with α/β and α domains in each monomer. It has ATPase activity in E. Coli to promote DNA strand separation, forming the open complex. Loop 1 (L1) and loop 2 (L2) are two loops locate in the α/β domains and α domains respectively. They are responsible for the interaction between PspF and the sigma 54. The 8 to 238 amino acids are the Sigma 54 interactive domain while the DNA strand binding motif is at the amino acid position 302 to 321. In our project, the original DNA-binding domain would be replaced by the Clp, leaving the transcription activation domain(TAD) in our biobrick design.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 90
Illegal AgeI site found at 118
Illegal AgeI site found at 256 - 1000COMPATIBLE WITH RFC[1000]
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