Coding

Part:BBa_K3165010

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-18)
Revision as of 21:14, 18 October 2019 by Registry (Talk | contribs)

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N-Terminal T7 RNAP Domain + nMag (Optimised for Escherichia coli)

This part consists of the N-Terminal T7 RNA Polymerase domain attached to a magnet domain (nMag) via a linker sequence. The T7 RNA Polymerase holoenzyme has been cleaved at specific sites to create two domains (the N-terminal T7 RNAP domain and the C-terminal T7 RNAP domain) both of which are individually linked to light-sensitive domains nMag and pMag via short linker sequences. On appropriate stimulation, the light-sensitive domains dimerize making the T7 RNA Polymerase functional again leading to the transcription of the genes under the T7 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 835
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1284


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