Coding

Part:BBa_K2984051

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 11:07, 17 October 2019 by Chlamy Dima (Talk | contribs)


L1c-PsaD-bleRscp-YFP-RbcS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a bleomycin resistance with self cleaving peptide scp, a B2-B2 linker, a YFP mVenus marker, and the Rbcs2 terminator. This Level 1 construct is designed for comparison of locus dependent expression through fluorescence intensity measuerments.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1833
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1833
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1833
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1833
    Illegal NgoMIV site found at 1453
    Illegal NgoMIV site found at 2310
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

colonies_total
Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii


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Categories
Parameters
None