Composite

Part:BBa_K3110029

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 18:37, 14 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Weak Promoter Medium RBS lldP+lldD

L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a weak promoter and a medium RBS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 329
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2859
    Illegal AgeI site found at 465
    Illegal AgeI site found at 928
    Illegal AgeI site found at 1366
    Illegal AgeI site found at 1525
    Illegal AgeI site found at 1537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2296


[edit]
Categories
Parameters
None