Composite

Part:BBa_K3110022

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 16:57, 14 October 2019 by Registry (Talk | contribs)

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Strong Promoter Strong RBS lldP+lldD

L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a strong promoter and a strong RBS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2864
    Illegal AgeI site found at 464
    Illegal AgeI site found at 927
    Illegal AgeI site found at 1365
    Illegal AgeI site found at 1524
    Illegal AgeI site found at 1536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2301


[edit]
Categories
Parameters
None