Composite

Part:BBa_K3110010

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 17:38, 18 October 2019 by Meghamariajacob (Talk | contribs)


Weak Promoter Strong RBS lldD

L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a strong RBS. lldD cleaves L-lactate to pyruvate. Weak promoter and strong RBS ensures low rate of lldD production. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1202
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 639


[edit]
Categories
Parameters
None