Coding
ARS B2-B2

Part:BBa_K2984000

Designed by: Sophia Schiebler   Group: iGEM19_Humboldt_Berlin   (2019-10-02)
Revision as of 13:47, 17 October 2019 by Chlamy Dima (Talk | contribs)


3'UTR, Secretion signal of Arylsulfatase ARS B2-B2

This vector is a part of the Chlamy-HUB-Collection. For the secretion of a protein in Chlamydomonas reinhardtii it is necessary that this protein is attached to a signal sequence that targets it to the secretory pathway and eventually into the medium. It has been demonstrated that the Arylsulfatase 1 (ARS1) is not only able to direct protein synthesis to the secretory pathway, it was also shown that it is able to secrete heterologous proteins (Rasala et al., 2012). The amino acid sequence of the signal peptide of ARS1 is MHARKMGALAVLAVACLAAVASVAHA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
regulator
Parameters
function