Composite

Part:BBa_K2963021:Design

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-12)
Revision as of 08:34, 12 September 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pgsBCA- encoding a poly-γ-glutamic acid synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2207
    Illegal PstI site found at 2188
    Illegal PstI site found at 3467
    Illegal PstI site found at 3753
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2365
    Illegal NheI site found at 2963
    Illegal NheI site found at 4254
    Illegal PstI site found at 2188
    Illegal PstI site found at 3467
    Illegal PstI site found at 3753
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2207
    Illegal PstI site found at 2188
    Illegal PstI site found at 3467
    Illegal PstI site found at 3753
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2207
    Illegal PstI site found at 2188
    Illegal PstI site found at 3467
    Illegal PstI site found at 3753
    Illegal NgoMIV site found at 2586
    Illegal AgeI site found at 4111
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to express PgsBCA complex heterologously in corynebacterium glutamicum, we add Ptac promoter with a short RBS before the start codon and T7 terminater of each protein， respectively.


Source

Bacillus Genome.

References