Part:BBa_K3228073

pMC_1_001_characterization_PRO_S.e.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 3503
  Illegal PstI site found at 6038
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 6038
  Illegal NotI site found at 2305
  Illegal NotI site found at 8042
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 7201
  Illegal XhoI site found at 3553
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 3503
  Illegal PstI site found at 6038
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 3503
  Illegal PstI site found at 6038
  Illegal NgoMIV site found at 2126
  Illegal NgoMIV site found at 2352
  Illegal NgoMIV site found at 2494
  
• 1000
  COMPATIBLE WITH RFC[1000]

Description

Enter part specific description here

either this picture Picture of the promoter and rbs entry vectors for measurement. or this picture Picture of the LVL 2 entry vector for measuring terminators (with YFP and mTurqoise)

This BioBrick is part of the measurement entry vectors for the characterization of BioBricks in cyanobacteria (Part range BBa_K3228073 to BBa_K3228075 as well as BBa_K3228090) by the iGEM Team Marburg 2019. They contain our MoClo compatible shuttle vector for cyanobacteria BBa_K3228069 and are therefore the only MoClo based vector for the characterization of BioBricks in cyanobacteria. These pre assembled LVL 1 plasmids contain a place holder for their respective BioBrick type that acts as a Dropout to quickly and effortlessly insert any part of the same type for an easy characterization. In our results LLLLink to resultsLLL we show how these measurement entry vectors can save a lot of effort and money when characterizing a greater library of parts. Additionally the usage of the same entry vector for each measurement will aid in greater comparability and reproducibility. For greater comparability across other data sets we decided to use te same Biobricks as in measuring the toolbox for Vibrio natriegens in the last year Vibrio natriegens in the last year.

Compability

These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.