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Composite
Part:BBa_K3040000
Designed by: TSAI, CHIA-TSEN Group: iGEM19_NTHU_Taiwan (2019-10-18)
Lipase A (Pseudomonas sp. 7323) -Histag
Lipase from Antarctic cold-tolerant Pseudomonas sp. 7323 can catalyze the hydrolysis of triacylgly-cerols to glycerol and monoacyl-glycerols. We utilize the characteristic of lipase-having different catalytic activity under different temperature, to sense the temperature change. Lipase A reaches its catalytic activity peak at about 30℃, and decreases as temperature rise or drop. Furthermore, to purify our lipase A in an easier way, we design a His-tag sequence behind the lipase A.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 376
Illegal NgoMIV site found at 488
Illegal NgoMIV site found at 1523 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |