Composite

Part:BBa_K3160011

Designed by: chi xu   Group: iGEM19_XHD-WS-Wuhan-A   (2019-10-15)
Revision as of 07:24, 15 October 2019 by Registry (Talk | contribs)

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pEGFP-miR196a-148a sensor

The expression of miR-196a and miR-148a were found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a miR-148a sensor contain two complementary binding sites to miR-196a miR-148a which inhibit miR-196a and miR-148aexpression. The sequence of miR-196a and miR-148asensor wrere synthetised by GenScript (Shanghai, China). We inserted miR-196a miR-148a sensor into the 3' end of GFP, which generated GFP- miR-196a miR-148a sensor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None