Part:BBa_K1678007
Vitamin B12 biosensor, composed by a cobalamin riboswitch in front of an mRFP1
This part can be used as a Vitamin B12 biosensor, we tested it for the AdoCobalamin form of B12 (AKA Coenzyme B12), but it might be used also to detect CyanoCobalamin, HydroxyCobalamin, MethylCobalamin according to the following publication : "A riboswitch sensor to determine vitamin B12 in fermented foods"
Xuan Zhu 2015. We didn't tried this because we were out of time, anyway this would have to be characterized.
2019 OUC-China's characterization and improvement
Background
Background of 2019 OUC-China's project——RiboLego
Due to context-dependent performance and limited dynamic range, the widespread application of riboswitches is currently restricted. By replacing its original ORF with a new one, the structure of an aptamer domain can be subtly disrupted, resulting in a loss of ligand response. So riboswitch is still not be considered as a ‘plug and play' device. To tackle these problems, our project focuses on a standardized design principle to be used for modular and tunable riboswitch. The modular riboswitch we defined consists of the original riboswitch, Stabilizer and Tuner. Stabilizer can protect the structure of riboswitch from damage while Tuner can reduce the expression probability of fusion protein and make improvement of riboswitch function.
Background of this part
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 964
Illegal AgeI site found at 1076 - 1000COMPATIBLE WITH RFC[1000]
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