Plasmid

Part:BBa_K2908681:Design

Designed by: Dong Yufan   Group: iGEM19_CSU_CHINA   (2019-10-17)
Revision as of 21:47, 17 October 2019 by Sqx98625 (Talk | contribs) (→‎Design Notes)


s(ESR1)p-miR101spe-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 155
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 200
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initiation codon ATG can only appear between miRNA sponse and EGFP.
- The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture. T--CSU_CHINA--ggmodule2.png

Source

We designed it by ourselves and constructed this part with the help of Golden gate.

References