Plasmid
Part:BBa_K2908671
Designed by: Dong Yufan Group: iGEM19_CSU_CHINA (2019-10-16)
pLN431-s(GATA3)p-GAD-miR101-BS
The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of GAD and miR101-BS for sequence-specific expression of GAD.Therefore,we used our p(GATA3)p(BBa_K2908000) as a specific promoter. At the same time we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation domain of GAD.In this way we can ensure the specificity and independence of this protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 360
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 279
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Categories
Parameters
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