![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3040514
Designed by: TSAI, CHIA-TSEN Group: iGEM19_NTHU_Taiwan (2019-10-16)
pFadD promoter with pLac regulating downstream RFP
Tes A is a heterologous thioesterase, which is able to hydrolyze fatty acyl-CoAs to free fatty acids. Here, we combine Tes A with pFadBA (wild type) together as a new promoter, Tes A pFadBA, to see if the sensitivity range of fatty acid concentration lowers due to the increase of endogenous fatty acid.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 30
Illegal suffix found in sequence at 230 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 30
Illegal SpeI site found at 231
Illegal PstI site found at 245
Illegal NotI site found at 36
Illegal NotI site found at 238 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 30
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 30
Illegal suffix found in sequence at 231 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 30
Illegal XbaI site found at 45
Illegal SpeI site found at 231
Illegal PstI site found at 245
Illegal AgeI site found at 863
Illegal AgeI site found at 975 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |