Composite

Part:BBa_K3040514

Designed by: TSAI, CHIA-TSEN   Group: iGEM19_NTHU_Taiwan   (2019-10-16)
Revision as of 17:32, 16 October 2019 by Registry (Talk | contribs)

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pFadD promoter with pLac regulating downstream RFP

Tes A is a heterologous thioesterase, which is able to hydrolyze fatty acyl-CoAs to free fatty acids. Here, we combine Tes A with pFadBA (wild type) together as a new promoter, Tes A pFadBA, to see if the sensitivity range of fatty acid concentration lowers due to the increase of endogenous fatty acid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 30
    Illegal suffix found in sequence at 230
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 30
    Illegal SpeI site found at 231
    Illegal PstI site found at 245
    Illegal NotI site found at 36
    Illegal NotI site found at 238
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 30
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 30
    Illegal suffix found in sequence at 231
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 30
    Illegal XbaI site found at 45
    Illegal SpeI site found at 231
    Illegal PstI site found at 245
    Illegal AgeI site found at 863
    Illegal AgeI site found at 975
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None