Coding
DSAOP

Part:BBa_K3098024

Designed by: Yiru Shen   Group: iGEM19_WHU-China   (2019-10-01)
Revision as of 04:56, 2 October 2019 by RiruShen (Talk | contribs)


DSAOP(Designed Anti-oxidant Peptides)

Free radicals, such as superoxide anions (O2·-) or hydroxyl radicals (HO·), can cause protein damage and DNA mutations that can initiate several diseases, including coronary heart diseases and diabetes. Antioxidants that can scavenge free radicals play an important role in biological systems.An antioxidant peptide derived from Pinctada fucata has great poteintial for scavenging free radicals. Howeverthe the low rates of enzymatic hydrolysis extraction and purification, as well as the higher costs for purifying from Pinctada fucata meat, suggest that this natural and safe antioxidant peptide would not meet industrialized production requirements. Thus we submit a designed AOP that can be easily produced and purified in E.coli, named 'DSAOP'.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 102
    Illegal BamHI site found at 145
    Illegal XhoI site found at 343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisE.coli BL21