Coding

Part:BBa_K3226019:Design

Designed by: Yuma matsumoto   Group: iGEM19_Botchan_Lab_Tokyo   (2019-10-16)
Revision as of 15:14, 17 October 2019 by Youma (Talk | contribs) (→‎Design Notes)


RecA+his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 242
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 916
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When PCR was performed, we were careful to use primers that insert a 6 × his tag between the start codon of RecA and the subsequent sequence.

Source

Deinococcus Radioduranse

References