Plasmid_Backbone
L1b
Part:BBa_K2984005
Designed by: Dimitri Schumacher Group: iGEM19_Humboldt_Berlin (2019-10-12)
Revision as of 13:51, 17 October 2019 by Chlamy Dima (Talk | contribs)
Codon-Usage-Optimized Paromomycin Resistance For Use in Chlamydomonas reinhardtii
This vector is a part of the Chlamy-HUB-Collection. The Level 1 plasmid “L1b” uses an ampicillin resistance for bacteria selection and its multiple cloning sites “RFP” is flanked by the Golden Gate restriction enzymes BsaI (GGAG/CGCT) and BpiI (GCAA/ACTA). In comparison to the original pICH47732 backbone, the “L1b” plasmid has a decreased size of more than 2000 bases.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 298
Illegal NotI site found at 122 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 13
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 401
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |