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Coding

Part:BBa_K2984065

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 20:28, 16 October 2019 by Registry (Talk | contribs)

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Carbonicanhydrase

Level 1 construct containing secretion signal of arylsulfatase fused to the flourescent protein mVenus. To influence the expression of mVenus, a bleomycin resistance gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the Psadintron. The construct ends with the Rbcs2 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 280
    Illegal PstI site found at 627
    Illegal PstI site found at 1000
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 280
    Illegal PstI site found at 627
    Illegal PstI site found at 1000
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 259
    Illegal XhoI site found at 445
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 280
    Illegal PstI site found at 627
    Illegal PstI site found at 1000
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 280
    Illegal PstI site found at 627
    Illegal PstI site found at 1000
    Illegal NgoMIV site found at 382
    Illegal NgoMIV site found at 1269
    Illegal AgeI site found at 1210
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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