Part:BBa_K2984048

L1c-PsaD-bleRscp-ARS-PETase-SP20-RbcS2

This part is composed of the PsaD promoter (BBa_K2984008), bleomycin resistance with self cleaving peptide scp (BBa_K2984045), the ARS secretion signal (BBa_K2984000), the PETase enzyme (), the secretion enhancing SP20 glycomodule (BBa_K2984015), and the Rbcs2 terminator (BBa_K2984018).

The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The bleomycin resistance leads to a higher expression of the enzyme, due to its working mechanism. For every bleomycin moleucle, the bleomycin resistance gene must be expressed once. The resistance works in a ratio of 1:1 to the antibiotic. By having the resistance in the same open reading frame, the enzyme is also expressed, leading to a higher expression of the enzyme.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2249
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4
  Illegal XhoI site found at 1973
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1607
  
• 1000
  COMPATIBLE WITH RFC[1000]